Journal: Molecules

Article Title: The Proteolytic Activity of Neutrophil-Derived Serine Proteases Bound to the Cell Surface Arming Lung Epithelial Cells for Viral Defense

doi: 10.3390/molecules29184449

Figure Lengend Snippet: MHC I cell surface expression increases after treatment with proteases. A549, H1299, and Jurkat cells were incubated with CatG [10 μg/mL], NE [10 μg/mL], or both for 6 h in PBS, and the MHC I cell surface expression was analyzed using flow cytometry. Data were normalized to isotype control and considered significant at p < 0.05 (*), p < 0.01 (**), or p < 0.001 (***) by using an unpaired one-way ANOVA and Sidak post hoc test. n = 3.

Article Snippet: The following cell lines were used in this study: A549 cells (CCL-185, American Type Culture Collection, ATCC, Manassas, VA, USA), an adenocarcinoma human alveolar basal epithelial cell line; NCI-H1299 cells (H1299, CRL-5803, American Type Culture Collection, ATCC, Manassas, VA, USA), a human epithelial-like, non-small cell lung carcinoma cell line derived from the lymph node; and Jurkat E6-1 cells (Jurkat, human T cell lymphoblasts, ARP-177, BEI Resources; NIAID, NIH, Bethesda, MD, USA [ ]).

Techniques: Expressing, Incubation, Flow Cytometry, Control

Journal: Clinical and Experimental Immunology

Article Title: Cultured lymphocytes’ mitochondrial genome integrity is not altered by cladribine

doi: 10.1093/cei/uxad112

Figure Lengend Snippet: Proliferation rates of the T-cell lines and effects of cladribine on cell viability. (a) The growth rate of Jurkat E6.1 is faster than the CCRF-CEM cell line ( n = 3). (b) A cladribine dose response curve in Jurkat E6.1 cell line for cell proliferation with 48 hours ( n = 5) and 96 hours culture ( n = 5). (c) The effect of cladribine on cell proliferation in Jurkat with 12 days ( n = 5) and 16 days in culture ( n = 5). (d) The effect of cladribine on cell proliferation in CCRF-CEM with 2 days ( n = 5), 8 days ( n = 4), and 12 days ( n = 4) in culture ( n = 5).

Article Snippet: The human leukemic Jurkat E6.1 cell line (pseudodiploid, modal No 46) and the CCRF-CEM (ECACC 85112105) cell line (2 n = 46) were obtained from the European Collection of Authenticated Cell Cultures (ECACC) general cell collection and cultured according to the recommended instructions (Jurkat E6.1: RPMI 1640; 2 mM glutamine; 10% FBS) (CCRF-CEM: RPMI 1640; 2 mM glutamine; 20% FBS).

Techniques:

Journal: Clinical and Experimental Immunology

Article Title: Cultured lymphocytes’ mitochondrial genome integrity is not altered by cladribine

doi: 10.1093/cei/uxad112

Figure Lengend Snippet: Effect of cladribine on mitochondrial protein synthesis. (a) Immunoblot of MT-ATP6, MT-CO1, MRPL11, MRPS35, CASPASE3, SDHA, and CANX after 12 days culture with cladribine. In this experiment, 10 nM cladribine in CCRF cells resulted in extensive cell death, and immunoblot was not performed. (b) A pulse metabolic labeling of mitochondrial protein synthesis. The effect on mitochondrial protein synthesis in the CCRF-CEM and Jurkat cells after treatment with cladribine for 8 days ( n = 3) or 12 days ( n = 3).

Article Snippet: The human leukemic Jurkat E6.1 cell line (pseudodiploid, modal No 46) and the CCRF-CEM (ECACC 85112105) cell line (2 n = 46) were obtained from the European Collection of Authenticated Cell Cultures (ECACC) general cell collection and cultured according to the recommended instructions (Jurkat E6.1: RPMI 1640; 2 mM glutamine; 10% FBS) (CCRF-CEM: RPMI 1640; 2 mM glutamine; 20% FBS).

Techniques: Western Blot, Labeling